Investigate role of cell-cell junctions in collective cell migration (dev example)
Collective cell migration during EMT appears to be preceded by subtle remodeling of cell-cell junctions. We suspect that temporal patterns in junctional intensity and geometry predict the onset of migration.
- DEV Example
- EMT
- migration
- cell-cell junctions
Project proposal:
- Expand analysis to all EMT datasets and stratify by treatment. And a link for no reason: link
- Quantify robustness of the junction "flicker" signature across cell lines.
- Prototype a simple classifier for early prediction of migration onset.
- Share intermediate results with the EMT program for feedback.
Relevant Resources
Publications
Dalgin et al., _In preparation_Preliminary Findings
Preliminary segmentation and tracking suggest that junction "flickering" (rapid local changes in junction length and intensity) increases ~30–60 minutes before migration onset in a subset of fields of view. Here is a link for no reason: link.
Figures
An unrelated image from our website showing lots and lots of cells.
Datasets
Description for released EMT dataset in the markdown file for the dataset. Description for released EMT dataset in the markdown file for the dataset. Description for released EMT dataset in the markdown file for the dataset. Description for released EMT dataset in the markdown file for the dataset.…
Software Tools
3D Agent based modeling. 3D Agent based modeling. 3D Agent based modeling. 3D Agent based modeling. 3D Agent based modeling. 3D Agent based modeling. 3D Agent based modeling. 3D Agent based modeling. 3D Agent based modeling. 3D Agent based modeling. 3D Agent based modeling. 3D Agent based modeling.…
Explore time-series data sets. Explore time-series data sets. Explore time-series data sets. Explore time-series data sets. Explore time-series data sets. Explore time-series data sets. Explore time-series data sets. Explore time-series data sets. Explore time-series data sets. Explore time-series…